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Journal of Entomological Research
Year : 2009, Volume : 33, Issue : 3
First page : ( 189) Last page : ( 193)
Print ISSN : 0378-9519. Online ISSN : 0974-4576.

Molecular detection of prevalence of an endosymbiont alpha-proteobacteria (Rickettsiae), Wolbachia in some agriculturally important insects from Punjab (India)

Sharma R.K.*, Sharma Smriti**, Singh Satnam1,***, Kumar Rajinder, Gupta V.K.

Department of Entomology, Punjab Agricultural University, Ludhiana - 141 004, Punjab, India.

1 Corresponding author's E-mail: drsatnamsingh@yahoo.co.in

* Zonal Research Station for Kandi Area, Ballowal Saunkhri, Nawashahar, Punjab.

** Deptt. of Plant Breeding, PAU, Ludhiana - 141 004, Punjab.

*** Regional Station, PAU, Faridkot, Punjab.

Accepted:  10  August,  2009.


Different insect species belonging to six orders collected from fields of Punjab Agricultural University, Ludhiana and adjoining areas from different hosts were tested for the prevalence of an endosymbiont alpha-proteobacteria, Wolbachia with PCR amplification using specific primers (10 individuals of each species). The maximum infection was observed in whitefly (80%) followed by T. dome and C. blackburni (70%). While it was 60 percent in dusky cotton bug and T. chilonis chilonis, 50 percent in ants, Trichogramma sp. (Faridkot isolates) and Apis sp., 40 percent in cotton jassid, cotton grey weevil and pea blue butterfly. The minimum infection (10%) was observed in rice leaf folder and rest all the other species were found to be Wolbachia negative. The overall prevalence of bacterial endosymbiont was about 42 percent in the studied insect species of the region. The studies further revealed two different strains of Wolbachia, associated with these insects with 53.8 and 46.1 percent prevalence of strain A and B, respectively.


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