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Journal of Biological Control
Year : 2011, Volume : 25, Issue : 4
First page : ( 337) Last page : ( 341)
Print ISSN : 0971-930X. Online ISSN : 2230-7281.

ITS sequencing of Indian isolates of Lecanicillium species

Ramanujam B.*, Balachander M., Roopa G., Rangeshwaran R., Karmakar Pritam

National Bureau of Agriculturally Important Insects, HA Farm Post, Bellary Road, Bangalore, 560024, Karnataka, India

* Corresponding author E-mail: bonamramanujam58@gmail.com

Online published on 20 February, 2012.

Received:  5  August,  2011; Revised:  16  September,  2011; Accepted:  21  October,  2011.

Abstract

The genetic diversity of thirty one isolates of Lecanicillium species isolated from insect hosts from various geographical regions of India were studied. Their phylogenetic relationships were determined using internal transcribed spacer, ITS1, ITS2 and 5.8S gene of rRNA sequence. Based on the sequence similarity of ITS region and construction of subsequent phylogenetic analysis (neighbour-joining method), 15 isolates were grouped as Lecanicillium lecanii, 11 isolates as L. attenuatum, 3 isolates as L. longisporum and 2 isolates as L. muscarium.

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Keywords

ITS region, Phylogenetic analysis, Lecanicillium sp.

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