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Journal of Animal Research
Year : 2019, Volume : 9, Issue : 4
First page : ( 597) Last page : ( 604)
Print ISSN : 2249-5290. Online ISSN : 2277-940X.
Article DOI : 10.30954/2277-940X.04.2019.16

Assessment of Incidence of Enterocytozoon hepatopenaei (EHP) in Penaeus vannamei in Maharashtra and Gujarat

Kurcheti Pani Prasad*, Mary S.J. Abisha Juliet, Dhayanath M.

Division of Aquatic Environment and Health Management, ICAR-Central Institute of Fisheries Education, Mumbai, India

*Corresponding author: PP Kurcheti; Email: kpaniprasad@cife.edu.in

Online published on 27 September, 2019.


The occurrence of Enterocytozoon hepatopenaei in Penaeus vannamei samples were collected from Maharashtra and Gujarat farms. In the present study, shrimp samples from various shrimp ponds from two districts of Maharashtra and two districts of Gujarat were collected over a period of one year (February 2016 to April 2017). A total of 4513 shrimp samples were assessed for the presence of EHP by molecular characterization. Out of shrimp samples analysed, 31.2% samples were positive for EHP. The screening of EHP was done by single step and nested PCR targeting spore wall protein gene (SWP) of EHP resulting in product size of 514 bp and 148 bp for EHP respectively.



Penaeus vannamei, EHP, Maharashtra, Gujarat and Nested PCR.


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