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Journal of Animal Research
Year : 2015, Volume : 5, Issue : 3
First page : ( 407) Last page : ( 413)
Print ISSN : 2249-5290. Online ISSN : 2277-940X.
Article DOI : 10.5958/2277-940X.2015.00071.6

Effect of Mitomycin-C Inactivation on Expression Pattern of Pluripotency Related Transcriptional Factors in Buffalo Fetal Fibroblasts and Wharton's Jelly

Parmar Mehtab S., Gupta Swati, Somal Anjali, Pandey Sriti, Chandra Vikash, Sharma G. Taru*

Reproductive Physiology Laboratory, Division of Physiology and Climatology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India

*Corresponding author: GT Sharma; Email: gts553@gmail.com

Online published on 19 November, 2015.

Abstract

Present study examined the effect of mitomycin-C (MMC) treatment on expression profile of pluripotency genes (Oct-4, Sox-2 and Nanog) in buffalo fetal fibroblasts (BFFs) and Wharton's jelly (BWJ) stem cells, generally used as a feeder cell support to grow the pluripotent stem cells. In a time dependent study, a variable response in relative mRNA expression of pluripotency genes was observed, expression of Oct-4 in BFF declined immediately post MMC inactivation but a significant elevation (P<0.05) was noticed later on. The relative mRNA expression remained unchanged in BWJ, up to 24 h post MMC treatment and thereafter, it increased significantly (P<0.05). A similar trend for the expression pattern of Sox-2 and Nanog was observed in both the cell types. MMC inactivation caused an upregulation in the expression of Sox-2 and Nanog in BFF while it remained unchanged (P>0.05) in BWJ. Results of this study suggests that MMC inactivation of the cells used as feeder support modulates the expression profile of pluripotency genes and this alteration in gene expression pattern is variable in different cell types.

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Keywords

Buffalo, feeder layer, Fetal fibroblasts, Wharton's jelly, mitomycin-C, Pluripotency genes.

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