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Indian Journal of Veterinary Pathology
Year : 2011, Volume : 35, Issue : 2
First page : ( 238) Last page : ( 240)
Print ISSN : 0250-4758. Online ISSN : 0973-970X.

Analysis of feed ingredients for Afla and T-2 mycotoxins by ELISA in rural areas of Uttar Pradesh

Rai R.B.*, Rahman Shafiqur, Dixit Himanshu, Rai Sweta, Singh Balvir, Kumar Harendra1, Damodaran T.2, Dhama K.

Division of Pathology, Indian Veterinary Research Institute, Izatnagar-243122

1Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar-243122

2C.S.S.R.I.-R.R.S., Lucknow (U.P.)

*Corresponding author: email: drrbrai@yahoo.co.in

Online published on 20 February, 2012.

Received:  20  January,  2011; Accepted:  25  July,  2011.


A survey of commonly available feed ingredients in rural households of Barabanki and Raebareli districts of Uttar Pradesh was conducted for the presence of afla and T-2 mycotoxins during winter months by commercially available ELISA kits. Out of 88 samples, comprising of 74 feed ingredients and 14 commercial poultry feed, 9% and 24% ingredient samples were negative for afla and T-2 toxins, respectively. Among the positive samples, 20% showed higher level of aflatoxins (20 ppb or more), whereas 35% samples had more than 100 ppb concentration of T-2 toxins. Generally, the problem of aflatoxin has been recorded more in the rainy season and thereafter, because of high relative humidity and optimum temperature. The present study highlighting the occurrence of two important mycotoxins (Aflatoxin and T-2) in feed ingredients of rural areas and the potential threat of mycotoxins on animal and human health requires an upsurge in the mycotoxin surveillance and monitoring even in colder seasons which would help timely follow up of suitable prevention and control strategies for alleviating the economic losses and reducing public health significance.



Aflatoxins, mycotoxins, T-2 toxins.


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