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Invertis Journal of Science & Technology
Year : 2016, Volume : 9, Issue : 2
First page : ( 79) Last page : ( 93)
Print ISSN : 0973-8940. Online ISSN : 2454-762X.
Article DOI : 10.5958/2454-762X.2016.00003.2

Screening marine bacterium strains of Actinomycetes as antagonists to pathogenic Vibrios, molecular identification of potent strain and media optimization by: Plackett-Burman analysis and response surface methodology

Neelanchal Vaid*, Sajeevan T.P.

National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Lakeside campus, Fine Arts Avenue, Cochin-682016, India

*E-mail: neelanchal.vaid@gmail.com

Online published on 18 May, 2016.

Abstract

Vibriosis is a major bacterial disease caused by Vibrio spp. afflicting all varieties of shrimps in culture at all stages and is a limiting factor in shrimp aquaculture practice worldwide. Marine Actinomycetes are bacterial in nature and have proven themselves as reliable sources of novel bioactive compounds against various aquatic pathogens. Methods: In the present study, fourteen isolates of marine Actinomycetes were screened for possible antagonistic properties against various pathogenic strains of Vibrio spp. Plackett-Burman screening and response surface methodology were employed to select and optimize a suitable fermentation medium for the selected potent strain. Based on bioactivity tests, most potent strain was subjected to molecular identification by 16SrRNA sequencing. Results: Screening was successfully done and a potent strain was selected as antagonistic to various Vibrio spp. The selected strain was identified as a member of the genus Nocardiopsis. Optimization conditions were successfully developed which ensured high yield of antibiotic production. Conclusion: The work opens up avenues for using Actinomycetes as antagonists to protect shrimps from Vibriosis. This has vital significance as it can lead to higher production of shrimps by bringing down high mortality rates caused by Vibriosis and thereby reducing huge financial losses incurred by aquaculture farmers.

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Keywords

Vibriosis, Marine Actinomycetes, Plackett-Burman analysis, Response surface methodology, Molecular identification.

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