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Indian Journal of Poultry Science
Year : 2012, Volume : 47, Issue : 1
First page : ( 36) Last page : ( 39)
Print ISSN : 0019-5529. Online ISSN : 0974-8180.

Standardization of site of in ovo injection, needle length, embryonic age and their effect on the hatchability of the egg and hatch weight

Bhattacharyya A.*, Majumdar S., Bhanja S.K., Mandal A.B.

Central Avian Research Institute, Izatnagar, Bareilly - 243 122 (U.P.)

*Corresponding author: Department of Poultry Science, DUVASU, Mathura-281001 (UP); e.mail - amitav16@rediffmail.com

Online published on 11 March, 2013.

Abstract

Two experiments were conducted for standardization of in ovo injection of nutrients and in ovo vaccination in turkey eggs. In the first experiment, 80 fertile eggs of small white variety were used for the in ovo injection of Indian ink @ 1 ml/egg with a 18 mm and 25 mm needle on 21st and 25th days of incubation at either broad or narrow end of the egg. The Indian ink injected eggs were broken immediately after injection to study the deposition sites. In the second experiment fertile eggs (n=377) of small white variety were injected in ovo with 10% glucose @ 1 ml/egg with 18 mm and 25 mm needle on 21st and 25th days of incubation at similar ends as mentained above. Two sham controls (21st and 25th days of incubation) were injected with 1 ml sterile water. Another group was maintained as un-injected control group. Injection with an 18 mm needle and 25 mm needle on 21st day of incubation at narrow and broad ends respectively resulted in deposition of Indian ink in allantoic cavity. When injection was carried out on 21st day and 25th day with a 25 mm needle at narrow end, deposition site was yolk sac and amniotic sac/yolk sac, respectively. Injection with an 18 mm needle at narrow end and 25 mm needle at broad end on 25th day resulted in Indian ink deposition at amniotic sac. Among in ovo glucose injected groups, percent hatchability was highest in the group in which injection was carried out with a 18 mm needle on 21st day at narrow end (94.44%) followed by the group consisting of eggs in ovo injected with a 25 mm needle at broad end on 25th day (80.56%). Further, it was observed that in ovo injection on 25th day resulted in higher hatchability% than 21st day (75.2 vs 60.5). When hatchability based on deposition site was compared, amniotic sac (77.26%) had highest hatchability. Chick weight to egg weight ratio was significantly (P< 0.001) higher on 21st day when injection was carried with a 25mm needle at narrow end (65.11%) and with a 25 mm needle on 25th day at broad end (63.97%). Further, it was found that injection of glucose on 21st day resulted in a higher chick weight to egg weight ratio compared to injection on 25th day (63.21 vs 62.35%). Hence, it can be concluded that in ovo injection of nutrients with a 25 mm needle on 21st day of incubation at narrow end may be carried out to improve chick weight.

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Keywords

In ovo injection, Indian ink, glucose, hatchability, turkey.

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