Effect Minimum Inhibitory Concentration and Minimum Bactericidal Concentration of Honey Bee Trigona spp on Streptococcus pyogenes
Streptococcus pyogenes one cause of respiratory tract infections including pharyngitis. Seventy-three percent of doctors prescribe antibiotics for pharyngitis. In developing countries, antibiotics are prescribed for 44–97% of hospitalized patients, sometimes with inappropriate doses. Improper antibiotic use can cause side effects such as allergies or diarrhea, increase health care costs, and increase the possibility of selection for antibiotic resistance. Research needs to be done on natural antibiotics found in natural ingredients. One of the natural ingredients empirically has many properties and is relatively safe is honey from bees. Honey has substances that are bactericidal and bacteriostatic like antibiotics. This study aimed Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) honey bee Trigona spp against Streptococcus pyogenes in vitro. In concentration of 2.5%, 5%, 7.5%, 10%, and 12, 5% and knowing the effect of honey bee Trigona spp with a concentration of 2.5%, 5%, 7.5%, 10%, and 12.5% against Streptococcus pyogenes in vitro. Type of research is the experiment with the Posttest-Only Control Group Design, the research material of honey from Trigona spp independent variable is the concentration of honeybees Trigona spp. The dependent variable is the level of turbidity produced in a tube to measure MIC and the number of colonies Streptococcus pyogenes on Blood Agar Plate media to measure MBC. The results of the MIC determination showed that all concentrations showed clear, MBC results the largest number of colonies occurred at a concentration of 2.5% of the total colony colonies and colonies growth the smallest occurred at a concentration of 10% of the number of colonies 0 colonies, Conclusions of MIC cone honey bee Trigona spp against bacteria Streptococcus pyogenes occurred at a concentration of 2.5%., MBC moth honey Trigona spp against bacteria Streptococcus pyogenes occurred at a concentration of 10%. There is an influence of the honey Trigona spp on the bacteria Streptococcus pyogenes, which is indicated by the value of p <0.05, which is 0, 000 using the Linear Regression test.
MIC, MBC, Streptococcus pyogenes.