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Indian Journal of Nematology
Year : 1987, Volume : 17, Issue : 2
First page : ( 241) Last page : ( 246)
Print ISSN : 0303-6960.

Sequential changes in proteins of soybean, inoculated with the root-knot nematode, Meloidogyne incognita

Simte H. C., Dasgupta D. R.

Division of Nematology, Indian Agricultural Research Institute, New Delhi-110 012.

Accepted:  March,  1987.

Abstract

The sequential changes in proteins of soybean were observed with an objective to establish the identity of buffer soluble enzyme proteins after inoculating with Meloidogyne incognita. Inoculated roots showed higher concentration of total buffer soluble protein over their respective controls (3,7, 14,21 and 28 days after inoculation). The ratio of inoculated/healthy was observed to be of high order (1.65), 14 days after inoculation. Disc-electrophoretic analysis showed that there were 3 additional bands which occurred 3–21 days after inoculation. The isozyme pattern of glucose-6-phosphate dehydrogenase was found to be the same in the healthy as well as in nematode inoculated root samples. Three anionic isozymes of malate dehydrogenase (MDH) were resolved on polyacrylamide gels from inoculated and healthy root samples. There was a pronounced increase in MDH activity in inoculated root samples over their control.

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Key words

M. incognita, soybean, Clark-63, Disc-electrophoresis, MDH, gl-6-PO4 dehydrogenase.

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