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Indian Journal of Mednodent and Allied Sciences
Year : 2017, Volume : 5, Issue : 1
First page : ( 30) Last page : ( 37)
Print ISSN : 2347-6192. Online ISSN : 2347-6206.
Article DOI : 10.5958/2347-6206.2017.00006.1

Evaluation of Satb1, Survivin, and Ki-67 as Possible Markers to Differentiate Phyllodes Tumour from Fibroadenoma

Ranade Ketan J1,**, Nerurkar Alka V2,*, Phulpagar Mayura D3,***, Shirsat Neelam V4,****, Shinde Sneha S5

1Former Ph.D. Student, Department of Biochemistry, T.N. Medical College, B.Y.L. Nair Hospital, Mumbai

2Professor and Ph.D. Guide, Department of Biochemistry, T.N. Medical College, B.Y.L. Nair Hospital, Mumbai

3Associate Professor, Department of Pathology, T.N. Medical College, B.Y.L. Nair Hospital, Mumbai

4Head, Department of Neuro Oncology, Advanced Centre for Treatment, Research and Education in Cancer (Tata Memorial Centre), Kharghar, Navi Mumbai

5Former M.B.B.S. Student, T.N. Medical College, B.Y.L. Nair Hospital, Mumbai

*Corresponding author email id: alka_nerurkar@rediffmail.com,

**k_ranade@yahoo.com/ranadeketan@gmail.com,

***mayuraph@yahoo.com

****neelamshirsat@hotmail.com

Online published on 29 April, 2017.

Abstract

Aims

Difficulties in diagnosis of phyllodes tumour are well known. Our aim was to observe whether the expressions of satb1, survivin, and ki-67 were helpful in differentiating fibroadenoma and phyllodes tumour.

Settings and design

Retrospective.

Methods and material

Paraffin embedded tissue samples from 60 female patients with phyllodes tumour and 60 female patients with fibroadenoma were studied. Expression of the gene products was studied and confirmed using immunohisto chemical and western blot analysis.

Statistical analysis used

The statistical analysis was performed using Fisher's exact test to find out the significant changes.

Results

Statistically significant difference was observed in Satb1, survivin expression between fibroadenoma and benign phyllodes cases. The difference between the Ki-67 expression in fibroadenoma and benign phyllodes cases was not statistically significant.

Conclusions

Our finding of strong stromal expression of satb1 and survivin in phyllodes as compared to fibroadenoma can be helpful for the development of additional diagnostic and prognostic indicators for otherwise difficult cases. Such immunochemical markers can be used to elucidate cellular basis of tumour behaviour. Validation of such immunochemical test in future will reduce diagnostic uncertainty in this rare tumour. In addition to that such parameters may serve as a therapeutic target that could increase effectiveness of chemotherapy or radiation therapy. A study with large number of samples along with clinical and follow-up data is required for confirmation.

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Keywords

Phyllodes, Satb1, Survivin, Ki-67, Fibroadenoma, Tumour, Benign Phyllodes, Of Chemotherapy, Radiation Therapy, Immunochemical markers.

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