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Indian Journal of Horticulture
Year : 2017, Volume : 74, Issue : 4
First page : ( 612) Last page : ( 614)
Print ISSN : 0972-8538. Online ISSN : 0974-0112.
Article DOI : 10.5958/0974-0112.2017.00118.9

Validation of molecular marker AVRDC-PP12 linked to male sterility gene ms10 of chilli

Aulakh Parjeet S., Dhaliwal M.S.*, Jindal S.K.

Department of Vegetable Science, Punjab Agricultural University, Ludhiana, 141004

*Corresponding author's E-mail: msdhaliwal696@gmail.com

Online published on 23 January, 2018.

Abstract

The newly identified SSR marker AVRDC-PP12 linked to the male sterility gene ms10 distinguished the heterozygous (Ms10ms10) fertile from the homozygous (Ms10Ms10) fertile plants in chilli segregating populations. To assess efficiency of AVRDC-PP12 in marker-assisted backcrossing for transfer of ms10 gene; the marker was screened in four backcross progenies. The marker co-segregated with ms10 gene in three progenies derived from the crosses ‘MS-12 × VR-16’, ‘MS-12 × S-217621’ and ‘MS-12 × Selection Dev’ with recombination frequency of 3.22, 4.16 and 3.27%, respectively. The marker failed to differentiate the parents in the cross ‘MS-12 × DCL-524’and hence was not used for further genotyping of the population.

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Keywords

Capsicum, nuclear male sterility, ms10, SSR markers.

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