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Indian Journal of Genetics and Plant Breeding (The)
Year : 2015, Volume : 75, Issue : 4
First page : ( 417) Last page : ( 425)
Print ISSN : 0019-5200. Online ISSN : 0975-6906.
Article DOI : 10.5958/0975-6906.2015.00068.1

Marker assisted pyramiding of major blast resistance genes Pi9 and Pita in the genetic background of an elite Basmati rice variety, Pusa Basmati 1

Khanna Apurva, Sharma Vinay1, Ellur Ranjith K., Shikari Asif B.2, Krishnan S. Gopala, Singh U. D.3, Prakash G.3, Sharma T. R.4, Rathour Rajeev5, Variar Mukund6, Prashanthi S. K.7, Nagarajan M.8, Vinod K. K.8, Bhowmick Prolay K., Rajashekhara H.9, Singh N. K.4, Prabhu K. V., Singh Ashok K.*

Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, 110 012

1Department of Bioscience and Biotechnology, Banasthali University, Banasthali, 304 022, Rajasthan

2Division of Genetics and Plant Breeding, Sher-E-Kashmir University of Agricultural Sciences and Technology of Kashmir, Srinagar, 191121, Jammu and Kashmir

3Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, 110 012

4ICAR-National Research Centre on Plant Biotechnology, New Delhi, 110 012

5Department of Agricultural Biotechnology, Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya, Palampur, 176 062, Himachal Pradesh

6Central Rainfed Upland Rice Research Station-ICAR-CRRI, Hazaribagh, 825 301, Jharkhand

7Agricultural Research Station, Mugad, University of Agricultural Sciences, Dharwad, 580 007, Karnataka

8Rice Breeding and Genetics Research Centre, ICAR-IARI, Aduthurai, 612 101, Tamil Nadu

9ICAR-Vivekanada Parvatiya Krishi Anusandhan Sansthan, Almora, 263 060, Uttarakhand

*Corresponding author's e-mail: aks_gene@yahoo.com

Online published on 9 November, 2015.

Abstract

Basmati is a premium quality rice of India which is highly priced in the international market. Pusa Basmati 1, an elite Basmati rice variety is highly susceptible to rice blast caused by Magnaporthe oryzae. Therefore, pyramiding blast resistance genes is essential to effectively combat the blast disease and increase the durability of resistance genes. The blast resistance genes Pi9 and Pita have been earlier demonstrated to be effective in Basmati growing regions of the country. Therefore, in the present study, monogenicnear isogenic lines Pusa 1637-18-7-6-20 and Pusa 1633-3-88-16-1 carrying Pi9 and Pita, respectively, were intercrossed to generate pyramided lines through marker assisted foreground, background and phenotypic selection for recurrent parent phenotype. The pyramided lines carrying Pi9+Pita were found to be either at par or superior to the recurrent parent Pusa Basmati 1 for agro-morphological, grain and cooking quality traits. Further, these pyramided lines were also found to show resistance against three virulent pathotypes of M. oryzae namely, Mo-nwi-kash 1, Mo-nwi-lon2 and Mo-ei-ran1, when evaluated under artificial inoculation conditions as well as in the natural epiphytotic conditions of uniform blast nursery at two locations. The developed pyramided lines are the potential sources of blast resistance genes in the Basmati improvement program and can also be released for commercial cultivation after required testing.

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Keywords

Basmati rice, blast resistance, gene pyramiding, marker assisted selection.

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