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Indian Journal of Genetics and Plant Breeding (The)
Year : 2012, Volume : 72, Issue : 3
First page : ( 380) Last page : ( 383)
Print ISSN : 0019-5200. Online ISSN : 0975-6906.

Agrobacterium-mediated genetic transformation of pigeonpea [Cajanus cajan (L.) Millisp] for pod borer resistance: Optimization of protocol

Sawardekar S. V.*, Mhatre N. K., Sawant S. S., Bhave S. G., Gokhale N. B., Narangalkar A. L., Katageri I. S.1, Kumar P. Ananda2

Plant Biotechnology Centre, College of Agriculture, Dapoli

1University of Agricultural Sciences, Dharwad

2National Research Centre on Plant Biotechnology, New Delhi

*Corresponding author's e-mail: svsawardekar@rediffmail.com

Online published on 25 September, 2012.

Abstract

Pod borer is a serious pest of pigeonpea. It was observed that borer infected flower buds do not produce any seed but only pods. In the present investigation, successful transfer and expression of cryIIAa gene was made through Agrobacterium into a popular pigeonpea variety, Konkan Tur-1. of pigeonpea through -mediated gene transfer method. The ability to regenerate in vitro as well use of positive selection system may provide means for recovery of transgenic pigeonpea.

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Keywords

Pigeonpea, transformation, pod borer, resistance.

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