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The Indian Journal of Genetics and Plant Breeding
Year : 1995, Volume : 55, Issue : 1
First page : ( 50) Last page : ( 57)
Print ISSN : 0019-5200.

Effect of genotypes and nutrient media on immature embryo culture of wheat

Agarwal D. K., Tiwari Sharad

Plant Tissue Culture Laboratory, Department of Plant Breeding & Genetics, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur 482004.

Received:  26  February,  1993; Accepted:  5  June,  1995.

Abstract

Immature embryo of 14 genotypes of Triticum aestivum and 4 genotypes of T. durum were cultured on four modified MS medium supplemented with 30 g.l−1 sucrose, 8 g.1−1 agarand different level of growth hormones, viz. 2 mg.l−1 2,4-D; 5 mg.l−1 2,4-D; 2 mg.l−1 2, 4-D + 100 ml.l−1 coconut milk; and 2 mg.l−1 2,4-D + 0.5 mg.l−1 kinetin. Highly significant differences in the response of genotypes, culture media and genotype X medium interactions were observed for callus initiation from immature embryos, formation of embryogenic calli and plantlet regeneration.

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Keywords

Embryo culture, genotype x medium interaction, wheat, T. aestivumT. durum.

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