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International Journal of Environmental Sciences
Year : 2010, Volume : 1, Issue : 4
First page : ( 656) Last page : ( 665)
Online ISSN : 0976-4402.

Optimization of the medium for the production of cellulase by the Trichoderma viride using submerged fermentation

Gautam S.P.1, Bundela P.S.2, Pandey A.K.3, Jamaluddin4, Awasthi M.K.2, Sarsaiya S.2,*

1Central Pollution Control Board, New Delhi, India.

2Regional office, M.P. Pollution Control Board, Vijay Nagar, Jabalpur (M.P.), India

3Mycological Research Laboratory, Department of Biological Sciences, Rani Durgavati University, Jabalpur (M.P.), India.

4Yeast and Mycorrhiza Biotechnology Laboratory, Department of Biological Sciences, Rani Durgavati University, Jabalpur (M.P.), India.

*Email: surendra_sarsaiya@yahoo.co.in

Online published on 3 January, 2012.

Abstract

The main purpose of this study to reduced the production cost of cellulase by using alternative carbon source such as municipal solid waste residue and optimized fermentation medium for high yielding. In the present investigation, to isolate the novel cellulase producing fungi, Trichoderma viride from municipal solid waste and to optimize the physicochemical and nutritional parameters for cellulase production. Municipal solid waste residue and yeast extract were found to be the best combination of carbon and nitrogen source for the production of cellulase by Trichoderma sp. Optimal concentration of municipal solid waste residue and yeast extract for the production of cellulase were 45% (w/v) and 1.0% (w/v), respectively. Optimum temperature and pH of the medium for the cellulase production by Trichoderma viride was 45 °C and 6.5. Cellulase production from Trichoderma viride can be an advantage as the enzyme production rate is normally higher as compared to other fungi.

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Keywords

Fermentation, optimization, biodegradation, cellulase, municipal solid waste.

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