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Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases
Year : 2007, Volume : 28, Issue : 1&2
First page : ( 45) Last page : ( 47)
Print ISSN : 0790-9320.

Genetic polymorphism between guinea fowl lines with high and low antibody response to sheep red blood cells using randomly amplified polymorphic DNA (RAPD) markers

Sharma D.*, Dhama K.

Genome Mapping Laboratory, Central Avian Research Institute, Izatnagar - 243 122, (UP).

*Corresponding author: Ds7758@yahoo.co.in

Abstract

A total of 20 random primers were used to detect genetic polymorphism between two divergently selected lines for antibody response to sheep red blood cell (SRBC) i.e. high antibody titre against SRBC (HSRBC) line and low antibody titre against SRBC (LSRBC) line in guinea fowl. All the 20 primers generated reproducible and distinct randmly amplified polymorphic DNA (RAPD) profiles and total number of bands ranged from 4 to 12 in the size range of 280 to 3600 bp. Two out of the 20 random primers (10%) could detect polymorphism between the HSRBC and LSRBC lines and total of 13 bands were amplified from these primers, out of which only 3 were polymorphic between the lines. The primers also differed in generating the extent of polymorphism within the group. Among the several polymorphic bands amplified one band of 1680 bp amplified with OPA18 seemed to be specific to HSRBC line.

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