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Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases
Year : 2006, Volume : 27, Issue : 2
First page : ( 97) Last page : ( 100)
Print ISSN : 0790-9320.

Genetic polymorphism between extreme responders to Ranikhet disease virus in chicken

Jackeray R., Raghuvanshi M., Sharma N., Tomar S.1, Dhama K.2, Sharma Deepak*

Avian Biotechnology Division, Central Avian Research Institute, Izatnagar - 243 122, (UP).

*Corresponding author.

1Vety. Officer (T-6), CARI, Izatnagar.

2Avian Disease Section, Division of Pathology, IVRI, Izatnagar.

Abstract

Genetic polymorphism within as well as between the high antibody titre (HAb) group and low antibody titre (LAb) group against Ranikhet disease (RD) virus was detected using Randomly Amplified Polymorphic DNA (RAPD) and Microsatellite/Minisatellite Associated Sequence Amplification (MASA) assay. Mean III titres (log 2n) in HAb and LAb groups were 7.27±0.14 and 1.66±0.17, respectively. The respective HI-GMT levels in HAb and LAb groups were 162.8±18.03 and 3.32±0.33, respectively. Four out of 16 random primers screened using pooled DNA samples for HAb and LAb groups, detected polymorphism between HAb and LAb groups. Using these 4 polymorphic primers, 8 group specific RAPD markers were identified. Out of these 8 markers, seven markers were specific to HAb group, while 1 marker was specific to LAb group. OPP03 400 marker was identified as fixed HAb specific marker. Two MASA primers were also used to detect the polymorphism between HAb and LAb lines. Though both the MASA primers amplified reproducible and distinct amplification profile, but none of these primers could detect polymorphism between HAb and LAb group.

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