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Indian Journal of Animal Research
Year : 2018, Volume : 52, Issue : 9
First page : ( 1331) Last page : ( 1336)
Print ISSN : 0367-6722. Online ISSN : 0976-0555.
Article DOI : 10.18805/ijar.v0iOF.9125

Identification of point mutation in TLR2 gene and its association with mastitis in water buffalo

Thakur Sunita1,*, Singh Mahendra2, Aseri Gajendra Kumar1, Verma Archana3, Vineeth M.R.3, Rayees Mohammad2, Arya Ashwani3

Dairy Cattle Physiology Division, ICAR-National Dairy Research Institute, Karnal-132 001, Haryana, India

1Amity Institute of Microbial Technology, Amity University, Jaipur-302 006, Rajasthan, India

2Dairy Cattle Physiology Division, National Dairy Research Institute, Karnal-132 001, India

3Dairy Cattle Breeding Division, National Dairy Research Institute, Karnal-132 001, India

*Corresponding author's e-mail: sunitathakurmicro@gmail.com,

Online published on 9 October, 2018.

Abstract

Mastitis is an economically important disease causing great loss to dairy sector owing to its negative effect on production. Its multi factorial etiology makes it difficult to use prophylactic methods for control. Identification of genetic resistance mechanisms and selection for it is a potential strategy for mastitis control. The present study was done in 101 Murrah buffaloes to find the common causative agents of mastitis in buffaloes, to identify variants in exon 2 and 3 ’UTR of TLR 2 gene and to study their association with intramammary infection. The microbiological examination revealed Staphylococcus aureus to be most common causative organism followed by Streptococcus agalactiae. PCR-RFLP using Sau3AI and HpyCH4V for exon 2 and 3 ’UTR respectively resulted in polymorphic pattern for exon2, while 3 ’UTR resulted in monomorphic pattern. PCR RFLP Sau3AI genotypes of TLR2 exon 2 were found to be highly associated with mastitis resistance. AAgenotype wasfound tobe least susceptible, while ABgenotype wasfound most frequent among affected buffaloes.

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Keywords

Buffalo, Mastitis, Murrah, TLR2, RFLP.

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