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Indian Journal of Agricultural Biochemistry
Year : 2013, Volume : 26, Issue : 1
First page : ( 86) Last page : ( 91)
Print ISSN : 0970-6399. Online ISSN : 0974-4479.

Effect of Sulfur and Nitrogen Nutrition on Storage Protein Quality in Mungbean [Vigna radiata (L.) Wilczek] Seeds

Kumar Arvind*, Sharma Sucheta, Sital J S, Singh Sarvjeet1

Department of Biochemistry, Punjab Agricultural University, Ludhiana-141004, India

1Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana-141004, India

*Author for correspondence: E-mail: arvind_ldl@rediffmail.com

Online published on 6 November, 2013.

Abstract

Field trials were conducted to observe the effect of sulfur and nitrogen nutrition on storage protein quality in mungbean seeds at maturity. Mungbean seeds of varieties PAU 911 and ML 818 were sown in the experimental fields. Soils were presupplemented with different sulfur and nitrogen sources either alone or in combinations @40 kg/ha. Treatments consisted of T1(control where neither sulfur nor nitrogen was added), T2(single super phosphate), T3(urea), T4(single super phosphate and urea), T5(gypsum), T6(ammonium nitrate) and T7(gypsum and ammonium nitrate). Different sources of sulfur and nitrogen either alone or in combinations significantly increased hundred seed weight, total nitrogen and sulfur content and decreased N:S ratio in PAU 911 and ML 818 seeds. The protein quality parameters i.e. crude protein, total soluble protein, sulfur containing amino acids (methionine and cysteine), various protein fractions i.e. albumin, globulin, glutelin and prolamins and sub-fractions of globulin i.e. legumin and vicilin were significantly increased under various treatments of sulfur and nitrogen. Among various treatments, T5 resulted in higher increase in the various protein quality parameters as compared to other treatments.

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Keywords

Mungbean, sulfur, nitrogen, storage protein fractions, seed quality.

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