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Indian Journal of Agricultural Biochemistry
Year : 2012, Volume : 25, Issue : 1
First page : ( 20) Last page : ( 24)
Print ISSN : 0970-6399. Online ISSN : 0974-4479.

Effect of Brassinolide and Salicylic Acid on Biochemical Parameters and Yield of Groundnut

Mandavia MK, Raval Lata1, Mandavia Chetana1, Karkar Chirag

Department of Biochemistry, Junagadh Agricultural University, Junagadh-362001; Gujarat, India

1Department of Agricultural Botany, Junagadh Agricultural University, Junagadh-362001; Gujarat, India

Online published on 11 July, 2012.

Received:  12  September,  2011; Accepted:  2  January,  2012.

Abstract

A field experiment was conducted to evaluate the response of Brassinolide and Salicylic acid on pod yield and biochemical parameters of groundnut leaves. Foliar spray of brassinolide @ 10 and 15 ppm and salicylic acid @ 300 and 500 ppm was done at vegetative (20 days after sowing) and reproductive (40 days after sowing) stages of groundnut variety GG 7 in kharif season. The results showed significant differences in pod yield and biochemical attributes viz., soluble protein, free amino acids, total DNA & RNA, nitrate reductase, polyphenol oxidase (PPO) and peroxidase activity between untreated and treated groundnut leaves. The pod yield increased significantly by treatment with both regulators sprayed at both stages; the maximum yield enhancement was observed in plants sprayed with 10 ppm brassinolide at reproductive stage. Between the two growth regulators, 500 ppm salicylic acid was more effective in case of free amino acid, peroxidase activity and PPO activity; whereas nitrate reductase was maximum with the spray of salicylic acid @ 300 ppm. Brassinolide @ 10 ppm was more effective in case of soluble protein, total DNA, while maximum increase of total RNA was recorded with brassinolide @ 15 ppm. Spraying of growth regulators at vegetative stage was better in terms of total DNA, free amino acid, peroxidase and nitrate reductase activity, whereas in case of soluble protein, total RNA and PPO activity, the spray at reproductive stage exerted more influence. Biochemical composition was improved in groundnut leaves in terms of increased content of metabolites studied. This, coupled with higher pod yield point out to the beneficial effect of brassinolide and salicylic acid.

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Keywords

Brassinolide, free amino acids, groundnut, nitrate reductase, peroxidase, pod yield, polyphenol oxidase, salicylic acid, soluble protein, total DNA, total RNA.

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