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Indian Journal of Agricultural Biochemistry
Year : 2005, Volume : 18, Issue : 2
First page : ( 83) Last page : ( 86)
Print ISSN : 0970-6399.

Antioxidant activity and nutraceutical potential of some soybean varieties

Singh Brahma N1, Barthwal Jyoti1, Upadhyay Garima1, Singh H.B.1, Prakash Dhan1,*, Singh R.L.2

1Nutraceutical Chemistry, National Botanical Research Institute, Lucknow-226001, India

2Department of Biochemistry, Dr R. M. L. Avadh University, Faizabad, India.

(*Corresponding author. Email: dprakash_in@yahoo.com)

Abstract

In order to find antioxidant activity and nutraceutical potential, seeds, leaves and pod pericarp of nine varieties of Glycine max were analysed for their total phenolic contents (TPC), antioxidant activity (AOA) and free radical scavenging activity (FRSA). TPC showed wide variation from 15.9 to 125.6 mg GAE/g and AOA was ranged from 11.2% (seeds, JS-355) to 82.1% (leaves, VLS-47). The leaf extracts of variety Indra Soya, NRC-2, PS-1042 and VLS-47 exhibited high amounts of TPC (91.6–125.6 mg GAE/g extract) and AOA (53.7–82.1%). In general, leaves of all the varieties were found to have higher TPC and antioxidant activity followed by seeds and pod pericarp. They were further subjected to FRSA assayed by DPPH free radical and expressed in terms of IC50 (inhibitory concentration) that ranged from 0.07 to 1.78 mg/ml, EC50 (efficiency concentration) from 3.0 to 77.3 mg/mg DPPH and ARP (antiradical power) from 1.2 to 32.9. The leaves of varieties VLS-47 showed the highest FRSA followed by Indra Soya and PS-1042 as evident by their low IC50 EC50 and high ARP values, than rest of the varieties. The reducing power expressed as ascorbic acid equivalent (ASE/ml) of leaf extracts of these varieties (1.1–1.5 ASE/ml) was found to be better than reference standard. The specific phenolic composition showed the presence of gallic acid, chlorogenic acid, ferulic acid, daidzein, genestein, kaempferol, quercetin and rutin. Among all the varieties studied, leaves of VLS-47 were found to possess higher gallic (72.5μg/g) and ferulic acids (47.2 μg/g). The results may be of importance for development of antioxidant nutraceuticals and bio-pharmaceuticals.

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Keywords

Glycine max, total phenolic contents, antioxidant activity, free radical scavenging activity, antiradical power, reducing capacity.

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