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Electronic Journal of Plant Breeding
Year : 2011, Volume : 2, Issue : 4
First page : ( 510) Last page : ( 519)
Online ISSN : 0975-928X.

Identification of contrasting genotypes for Fusarium wilt disease in Mucuna pruriens germplasm through combined in vitro screening and AFLP analysis

Sathyanarayana Mahesh SN*

Department of Biotechnology, Sir M Visvesvaraya Institute of Technology, Yelahanka, Bangalore-562 157

* E-mail: sathya_dixit@yahoo.com

Online published on 12 April, 2012.

Received:  09  August,  2011; Accepted:  30  November,  2011.

Abstract

Fifteen germplasm accessions identified on earlier field data were screened for Fusarium wilt resistance using in vitro culture filtrate approach. Infection and disease assessment were carried out based on wilt incidence and percentage mortality of the plants. Overall screening results revealed that 5 out of 15 accessions were moderate to highly resistant while 6 accessions were highly susceptible to Fusarium wilt. The results were further supported by differences in root and shoot weight and length amongst susceptible and resistant genotypes. Polymorphism analysis based on AFLP data revealed only a sub-set of these divergent genotypes confirming to genetic divergence levels required for raising segregant population. Based on the results of combined analyses, the genotypes 500108KA and IC471870, 500101KA and 500155AP have been recommended as suitable parents for developing population for mapping Fusarium wilt resistance in Velvetbean.

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Keywords

Mucuna pruriens var. utilis, Fusarium wilt, disease screening, AFLP, contrasting genotypes.

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