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Current Trends in Biotechnology and Pharmacy
Year : 2007, Volume : 1, Issue : 1
First page : ( 70) Last page : ( 78)
Print ISSN : 0973-8916. Online ISSN : 2230-7303.

Characterization of Myostatin Gene and Identification of SNPs for Diversity Analysis

Kumar S. T. Bharani1,2, Dilbaghi Neeraj2, Ahlawat S P S3, Mishra Bina1, Tantia M. S.1,*, Vijh R. K.2

1National Bureau of Animal Genetic Resources, Karnal, 132001, Haryana, India.

2Guru Jambheshwar University of Science and Technology, Hisar, HaryanaIndia

3Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India.

* For corresepondance- mstantia@yahoo.com

Abstract

In this present study we have sequenced the complete Myostatin gene with 3 exons, two introns, promoter region, 5’ and 3’ UTR region. We sequenced the entire gene by designing a set of 20 primers. Ten individuals from 5 breeds of Indigenous chicken were sequenced and the sequences obtained were aligned to develop a consensus sequence. We compared the sequences obtained among themselves, with Red Jungle Fowl and Chinese fowl. We estimated three genetic distances and constructed the phylogenetic tree using NJ method. The indigenous chicken joined together first followed by Red Jungle Fowl while the Chinese fowl was distinct. The indigenous gene sequence was similar among the various chicken breeds and might have been evolved from RJF following a separate course than the evolution of Chinese chicken.

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