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BIOINFOLET - A Quarterly Journal of Life Sciences
Year : 2013, Volume : 10, Issue : 3a
First page : ( 782) Last page : ( 784)
Print ISSN : 0973-1431. Online ISSN : 0976-4755.

Pollen viability assays in protogyny and self-incompatibility system in Indian mustard [Brassica juncea (L.) Czern. & Coss]

Chandrashekar U.S, Prasad Manjunath, Chakrabarty S. K, Dadlani M., Vishwanath K.*

Division of Seed Science and Technology, Indian Agricultural Research Institute, New Delhi-110012, India

*Department of Seed Science and Technology, UAS, GKVK, Bangalore-560065, India

Online published on 12 August, 2013.


Pollen viability studies on different staining methods viz., acetocarmine, FDA and 1% TTC were carried out in Indian mustard (Brassica juncea). The results differed significantly among the methods; acetocarmine recorded 74.47% pollen viability, followed by 42.42% and 20.15% by fluorescein diacetate (FDA) and triphenyl tetrazolium chloride (TTC) methods respectively. Pollen grains showed maximum viability on the day of stigma exertion (55.87%), which reduced to 52.21, 50.25, 36.26, and 34.07% after one, two, three and four days of stigma exertion, respectively.



Indian mustard, pollen viability, carmine, FDA, TTC.


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