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Agricultural Reviews
Year : 2016, Volume : 37, Issue : 1
First page : ( 55) Last page : ( 60)
Print ISSN : 0253-1496. Online ISSN : 0976-0741.
Article DOI : 10.18805/ar.v37i1.9265

Molecular techniques in rumen biotechnology: A review

Preedaa Manju G.1,*, Parkunan Thulasiraman2, Kumar Dhinesh R.2, Ramavathi J., Yazhini P., Sheikh Aasif Ahmad2, Dar Mohammad Rayees2, Priyadarshini Lakshmi2, Baghel Gunjan2, Chandrasekar T.2

Veterinary College and Research Institute, Namakkal-637 002, India

1Veterinary College and Research Institute (VC&RI), Tamil Nadu Veterinary and Animal Sciences University (TANUVAS), Namakkal, Tamil Nadu-637002

2ICAR-National Dairy Research Institute (NDRI), Karnal-132 001, India

*Corresponding author's e-mail: preprand@gmail.com

Online published on 17 March, 2016.

Abstract

This review analyses the use of molecular techniques in rumen microbial identification. The use of regular methods like roll tube and most probable number resulted in under estimation of rumen microbial growth. So molecular biology acts as an advanced tool for rumen microbial culture and identification of various new species. These techniques give complete descriptions of individual ruminal populations. Use of molecular techniques like PCR, DDGE and FISH, which also pave pathway for genetic manipulation of rumen microbes in the field of rumen manipulation. So the combination of traditional and molecular assays gives accurate and satisfactory results.

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Keywords

DNA, Microbes, Molecular methods, PCR, Rumen, RNA.

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