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Animal Nutrition and Feed Technology
Year : 2013, Volume : 13, Issue : 3
First page : ( 477) Last page : ( 487)
Print ISSN : 0972-2963. Online ISSN : 0974-181X.

Synergistic Effect of Cellulase and Xylanase on In Vitro Rumen Fermentation and Microbial Population with Rice Straw as Substrate#

Mao H.L.1,2, Wu C.H.1, Wang J.K.1, Liu J.X.1,*

Institute of Dairy Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou-310058, P.R. China

1MoE Key Laboratory of Molecular Animal Nutrition, Institute of Dairy Science, College of Animal Science, Zhejiang University, Hangzhou 310058

2College of Animal Science and Technology, Zhejiang A & F University, Lin'an-311300, P.R. China

*Corresponding author: liujx@zju.edu.cn

#This paper is part of the special issue entitled: Exogenous Enzymes in Animal Nutrition - Benefits and Limitations, Guest Edited by A.Z.M. Salem and N. Odongo, and Editor for Animal Nutrition and Feed Technology, A.K. Pattanaik.

Online published on 4 January, 2014.

Abstract

The in vitro gas test was conducted to investigate the effects of cellulase (CEL) and xylanase (XYL) on in vitro rumen fermentation and microbial population with rice straw as substrate. Three levels of CEL (0, 7.5 and 15 U/g of DM) and XYL (0, 15 and 30 U/g of DM) were tested in a 3×3 factorial arrangement. Addition of CEL and XYL could affect the gas production parameters. Methane emission was increased linearly by CEL addition (P<0.01), while the methane production was greater when XYL added at the intermediate dose (quadratic, P<0.01). Total volatile fatty acids (CEL, quadratic, P<0.01; XYL, linear and quadratic, P<0.05) were enhanced by either CEL or XYL addition. Ammonia nitrogen was reduced by CEL (linear and quadratic, P<0.05), while microbial protein was enhanced by addition of CEL (quadratic, P<0.01) and XYL (linear and quadratic, P<0.01). In vitro degradability of DM and NDF were increased by addition of CEL (quadratic, P<0.05) or XYL (quadratic, P<0.01). Addition of CEL increased the copy number of total bacteria (linear, P<0.01) and Fibrobacter succinogens (linear and quadratic, P<0.05). The outcome of this research indicated that the application of CEL and XYL could improve rumen fermentation, increase rice straw digestion and affect the rumen microbial population. Combination of CEL and XYL at the middle level is more effective than a single CEL or XYL.

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Keywords

Cellulase, Microbial population, Rice straw, Rumen fermentation, Xylanase.

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