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Asian Journal of Research in Pharmaceutical Science
Year : 2017, Volume : 7, Issue : 3
First page : ( 135) Last page : ( 140)
Print ISSN : 2231-5640. Online ISSN : 2231-5659.
Article DOI : 10.5958/2231-5659.2017.00022.4

A New Simultaneous HPLC Analytical method for Quantification of Benazepril Hydrochloride and its related Impurities in Bulk Drug Product

Rao Tentu Nageswara1,*, Apparao Karri1, Krishnarao N.1, Vijayalakshmi A.2

1Department of Chemistry, Krishna University, Machilipatnam, Andhra Pradesh, India.

2Department of Marine Living Resources, Andhra University, Visakhapatnam, Andhra Pradesh, India.

*Corresponding Author E-mail: tnraochemistry@gmail.com

Online published on 17 October, 2017.

Abstract

A simple and inexpensive method was developed with high performance liquid chromatography with PDA detection for determination of benazepril hydrochloride and its related impurities. The chromatographic separations were achieved on (250×4.6 mm), 5.0 μm make: Symmetry Shield column employing 0.02M tetrabuthylammonium hydroxide + 0.05% v/v acetic acid: methanol in the ratio of 50: 50 (v/v) as mobile phase with isocratic at flow rate 1.0mL/min was chosen. All impurities were eluted within 30 minutes. The column temperature was maintained at 25°C and a detector wavelength of 240 nm was employed. The method was successfully validated by establishing System Suitability, Specificity, Linearity, Precision, Accuracy, Limit of detection and Limit of quantification.

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Keywords

HPLC, Method validation, related impurities, Benazepril hydrochloride, LOQ, LOD.

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