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Asian Journal of Research in Pharmaceutical Science
Year : 2016, Volume : 6, Issue : 2
First page : ( 129) Last page : ( 134)
Print ISSN : 2231-5640. Online ISSN : 2231-5659.
Article DOI : 10.5958/2231-5659.2016.00018.7

Effects of Various Solvent on the Extraction of Antimicrobial, Antioxidant Phenolics from the Stem Bark of Decalepis hamiltonii Wight and Arn.

Samydurai P1,*, Saradha M.2

1PG and Research, Department of Botany, Kongunadu Arts and Science College (Autonomous), Coimbatore-641029, Tamil Nadu, India

2Department of Botany, Nirmala College for Women (Autonomous), Coimbatore-641018, Tamil Nadu, India

*Corresponding Author E-mail: samydurai.bio@gmail.com

Online published on 21 June, 2016.

Abstract

The present study aimed to investigate the effect of various solvent (petroleum ether, methanol and aqueous extracts) on the extraction of antimicrobial and antioxidant phenolics from stem bark extract of Decalepishamiltonii. The bark extracts were evaluated to find out photochemical by standard methods. Total content of phenolic compounds was estimated by Folin-Ciocalteau method. The susceptibility of microbial strains against the three extracts was determined using the well diffusion method. Antioxidant activity was assessed by DPPH and ABTS•+ assay. The results revealed that the methanol extract had more Phytochemical and highest phenolic content. The extract was exhibited highest zone of inhibition against tested pathogens and was the most potent scavenger of DPPH and ABTS•+ radicals. This study clearly indicated that the methanol solvent is the most suitable for extraction of antimicrobial and antioxidant phenolics from stem bark of D. hamiltonii and provide a better way to utilize in food and pharmaceutical industry.

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Keywords

Decalepis hamiltonii, total phenolics, antimicrobial, antioxidant activity, methanol extract.

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