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Asian Journal of Research in Chemistry
Year : 2015, Volume : 8, Issue : 1
First page : ( 27) Last page : ( 29)
Print ISSN : 0974-4169. Online ISSN : 0974-4150.
Article DOI : 10.5958/0974-4150.2015.00006.1

A Novel RP-HPLC Method for Determination of Lisinopril (LP) in Pure and Pharmaceutical Formulation

Latha Y.B. Manju1,*, Sankar D. Gowri2

1Sri Vasavi Institute of Pharmaceutical Sciences, Tadepalligudem-534 101. (A.P), India

2University College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, (A.P), India

*Corresponding Author E-mail: blessythall@gamil.com

Abstract

A simple, rapid and accurate and stability indicating RP-HPLC method was developed for the determination of LP in pure and tablet forms. The method showed a linear response for concentrations in the range of 20–100 μg/mL using methanol: Phosphate Buffer solution in the ratio (50:5 0) as the mobile phase with detection at 215 nm and a flow rate of 1.0 mL/min and retention time 2.611 min. The value of correlation coefficient, slope and intercept were, 0.999, 67074.3 and 18126.355, respectively. The method was validated for precision, recovery, ruggedness and robustness. The drug undergoes degradation under acidic, basic, peroxide and thermal degradation conditions. All the peaks of degraded product were resolved from the active pharmaceutical ingredient with significantly different retention time. As the method could effectively separate the drug from its degradation product, it can be employed as a novel stability indicating one.

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Keywords

LP, RP-HPLC, Degradation studies.

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