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Asian Journal of Research In Chemistry
Year : 2013, Volume : 6, Issue : 3
First page : ( 248) Last page : ( 253)
Print ISSN : 0974-4169. Online ISSN : 0974-4150.

Development and Validation of HPTLC Method for Quantification of Gallic acid and Catechin from Draksharishta

Tiwari Preeti1,*, Sen D.J.2, Patel Rakesh K.3

1Department of Pharmacognosy, Shri Sarvajanik Pharmacy College, Mehsana-384001, Gujarat, India

2Department of Pharmaceutical Chemistry, Shri Sarvajanik Pharmacy College, Mehsana-384001, Gujarat, India

3Head, Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat, India

*Corresponding Author E-mail: preetitiwari198311@yahoo.com

Online published on 6 May, 2013.

Abstract

A simple, precise and accurate HPTLC method has been established for the determination of gallic acid and catechin in test formulations of Draksharishta as Draksharishta-T and Draksharishta-M prepared by traditional and modern method respectively and also in its marketed formulation. Draksharishta is a polyherbal hydro alcoholic preparation and is used to improve digestion, as blood purifier, in the treatment of anaemia and advised as a choice of remedy in respiratory problems. The developed HPTLC method was validated in terms of precision, accuracy, LOD, LOQ and specificity. The amount of gallic acid in Draksharishta-T, M and in its marketed formulation was found to be 0.0227, 0.0225 and 0.0226% w/w respectively while catechin was found to be 0.0176, 0.0175 and 0.0175%w/w respectively. Quantification of gallic acid and catechin in Draksharishta has been reported first time by validated HPTLC. Furthermore, no TLC densitometric methods have been reported for the quantification of gallic acid and catechin from Draksharishta.

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Keywords

Draksharishta, HPTLC, validation, gallic acid, catechin.

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