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Asian Journal of Research In Chemistry
Year : 2012, Volume : 5, Issue : 6
First page : ( 759) Last page : ( 764)
Print ISSN : 0974-4169. Online ISSN : 0974-4150.

Development and Validation of HPTLC Method for Quantification of Gallic acid and Ellagic acid in Ashwagandharishta

Tiwari Preeti1,*, Patel Rakesh K.2

1Department of Pharmacognosy, Shri Sarvajanik Pharmacy College, Mehsana-384001, Gujarat, India

2Head of Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat, India

*Corresponding Author E-mail: preetitiwari198311@yahoo.com

Online published on 7 February, 2013.

Abstract

Ashwagandharishta is a polyherbal hydro-alcoholic formulation and is used as immunomodulator to promote the health and longevity by increasing defence against disease and also known for its usefulness in the treatment of hypercholesterolemia. A simple, precise, robust and accurate HPTLC method has been established for the determination of gallic acid and ellagic acid in Ashwagandharishta–T and Ashwagandharishta-M prepared by traditional and modern methods respectively and also in its marketed formulation. The developed HPTLC method was validated in terms of precision, accuracy, LOD, LOQ and specificity. The amount of gallic acid in Ashwagandharishta-T, M and its marketed formulation was found to be 0.0281, 0.0279 and 0.0280% w/w respectively while ellagic acid was found to be 0.0191, 0.0189 and 0.0188% w/w respectively. This is the first report for quantification of gallic acid and ellagic acid in Ashwagandharishta by HPTLC. Furthermore, no TLC densitometric methods have been reported for the quantification of gallic acid and ellagic acid from Ashwagandharishta.

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Keywords

Ashwagandharishta, HPTLC, Validation, Gallic acid, Ellagic acid.

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