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Asian Journal of Research In Chemistry
Year : 2012, Volume : 5, Issue : 2
First page : ( 193) Last page : ( 196)
Print ISSN : 0974-4169. Online ISSN : 0974-4150.

HPTLC methods for quantification of gallic acid in Bhuvneshvara vati for routine quality control

Jain V.1,*, Daharwal S.J.1, Jain Tripti2, Saraf Swarnlata1, Saraf S.1

1University Institute of Pharmacy, Pt. Ravi Shankar Shukla University, Raipur (C.G.), 492 010

2Chhattisgarh Food and Drug Administration, Raipur (C.G.) 492 010

*Corresponding author: vj_rsofiop@rediffmail.com

Online published on 7 February, 2013.

Abstract

Bhuvneshvara vati is one of the popular Ayurvedic preparations, official in Ayurvedic formulary of India. The present study is an attempt to develop a fingerprint method for Bhuvneshvara vati HPTLC with Method using gallic acid as a standard. The gallic acid is a major content in all the five herbal ingredients of the formulation. The method was validated for linearity, accuracy, limit of detection, limit of quantification, inter-day and intra-day assay precision, repeatability of measurement, and repeatability of sample application. The concentration of gallic acid present in raw material was found to be 3.10 ±0.41% w/w in emblica officinalis, 8.47±0.82% w/w in terminalia belerica, 4.63±0.49% w/w in Terminalia chebula, 1.82±0.73% w/w in Aegle marmelous and 0.26±0.65% w/w in Trichyspermum ammi. Gallic acid content in three identical laboratory batch of Bhuvneshvara vati BV-I, BV-II and BV-III, is found to be 2.61±0.468%, 2.59±0.567% and 2.63±0.392% w/w respectively with mean value 2.61±0.476%. The gallic acid content in all the three different batches is found to be in close proximities with each other.The results were comparable to marketed formulations.

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Keywords

HPTLC, Gallic acid, Bhuvneshvara vati, Ayurvedic formulation, Fingerprinting.

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