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Asian Journal of Research in Chemistry
Year : 2020, Volume : 13, Issue : 2
First page : ( 141) Last page : ( 145)
Print ISSN : 0974-4169. Online ISSN : 0974-4150.
Article DOI : 10.5958/0974-4150.2020.00028.0

Simultaneous Spectrophotometric estimation of Amoxicillin and Cloxacillin in their Pharmaceutical Dosage Form

Ghosh Moni, Dey Suddhasattya, Chatterjee Avijit, Sarkar Saptarshy, Khawas Sourav*

Dr. B. C. Roy College of Pharmacy and Health Sciences, Durgapur-713206, West Bengal, India

*Corresponding Author E-mail: souravkhawas497@gmail.com

Online published on 7 April, 2020.

Abstract

A new, simple, rapid and novel spectrophotometric method has been developed for estimation of Amoxicillin (AMX) and Cloxacillin (CLX) in bulk and combined pharmaceutical formulations using absorbance correction method (ACM). λ max of AMX was found at 209nm and CLX was at 249nm respectively. This method involved measurement of absorbance at two wavelengths of AMX and CLX i.e., 209 nm for AMX and 249nm for CLX. The combination is also estimated by ACM. It showed linearity of both the drugs at one wave lengths. Calibration curve was constructed at 209nm for AMX and 249nm for CLX respectively. Beer's law obeyed in concentration range of 10‐ 50μg/mL for AMX and 10–50μg/mL for CLX respectively by the method. These methods were validated for precision, reproducibility, linearity and accuracy as per ICH guidelines. The proposed methods are recommended for routine analysis since they are rapid, simple, accurate, cost effective and also sensitive and specific. It involves neither heating nor use of any organic solvent for separation of the combination. This study thus exploits the possibility for determining Pharmacokinetic data of the combined formulation which may be required in pre-clinical and clinical study in near future.

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Keywords

Amoxicillin, Cloxacillin, UV Spectroscopy, Validation.

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