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Asian Journal of Research in Chemistry
Year : 2020, Volume : 13, Issue : 2
First page : ( 113) Last page : ( 116)
Print ISSN : 0974-4169. Online ISSN : 0974-4150.
Article DOI : 10.5958/0974-4150.2020.00023.1

Simultaneous Estimation of Paroxetine hydrochloride and Clonazepam in Tablet Dosage form by HPLC

Gadge Sonali S.1,*, Game Madhuri D.2, Salode Vikrant L.1, Nakod Aashish D.1, Gadge Mahesh S.1

1P R Patil Institute of Pharmacy, Talegaon (SP), Dist-Wardha (MS) India-442202

2Vidybharati College of Pharmacy, C.K. Naidu road, Camp, Amravati (MS) India-444602

*Corresponding Author E-mail: sonaligadge94@gmail.com

Online published on 7 April, 2020.

Abstract

The main aim of this study was focuses on the simultaneous estimation of Paroxetine hydrochloride and Clonazepam in tablet dosage form by HPLC. The important objective was to develop and validate the method for accuracy. A simple, new and correct high performance liquid action technique was developed for coinciding determination of Clonazepam and Paroxetine in tablet dosage form by employing a C18 Intersil 250 × 4.6 millimeter column and mobile section consisting of Acetonitrile: 0.1% OPA (65: 35 v/v). The analysis was performed at a flow rate of 0.8 ml/min. The method was linear over the concentration range 75–375 μg/ml and 3–15 μg/ml for Paroxetine and Clonazepam respectively with limits of detection of 2.99, 0.1464 and limit of quantification of 9.0791, 0.4438 for Paroxetine and Clonazepam respectively. The average % recovery was found to be 101.61 ±1.31 and 100.03± 1.15 for Paroxetine and Clonazepam respectively. The retention times (tR) of Paroxetine and Clonazepam were found to be 4.7667 and 7.6667 respectively. The correlation coefficient for both the drugs were found to be 0.999. The method was validated in terms of accuracy, precision, ruggedness, linearity and range. The developed method is specific, rapid, specific, cheap which is suitable for the estimation of Paroxetine hydrochloride and Clonazepam in tablet dosage form.

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Keywords

Paroxetine hydrochloride, Clonazepam, High performance liquid chromatography, method validation, chromatogram.

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