Phytochemical Evaluation of Chloris barbata by using various Spectroscopic Techniques
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The present study was carried out to characterize the bioactive constituents present in methanol extracts of Chloris barbata using UV-VIS, FTIR and GC-MS. The crude extract was scanned in the wavelength ranging from 200–800 nm by using Perkin Elmer spectrophotometer and the characteristic peaks were detected. The FTIR spectrum confirmed the presence of hydrogen bonded acids, phenols, alcohols, amines, alkanes, alkenes, alkynes, aromatics, aldehydes and nitriles in a methanol extract of Chloris barbata. For GC-MS analysis, 10 g sample was extracted with 30 ml methanol, filtered in ash less filter paper with 2 g sodium sulphate and the extract is concentrated to 1 ml by bubbling nitrogen into the solution. The compound detection employed the NIST Ver.2.0 Year 2005 library. The biological activities are based on Dr. Duke's phytochemical and ethno botanical Databases by Dr. Jim Duke of the Agricultural Research Service/USDA. The UV-VIS profile showed different peaks ranging from 200–800 nm with different absorption respectively. The results of the GC-MS analysis provide different peaks determining the presence of 6 phytochemical compounds with different therapeutic activities. The major phytochemical constituents were 2-carboxymethyl-3-methyl-cyclopentane carboxylic acid (11.57%), acetic acid,3,3,6-trimethyl-4-oxo-3,4-dihydro-2H-pyran-2-yl-ester (3.04%), ursodeoxycholic acid (33.58%), 1-octene, 2-methoxy (2.8), 2-propenimine,3-[1-cyclohexenyl]-N-cyclohexyl, N-oxide (40.4%) and 1,3,12-Nonadecetriene (7.8%). Hence, this study offers base of using as herbal alternative for the synthesis of anti cancer agents.
Evaluation, chlorisbarbata, herbal, plant and stem.