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Asian Journal of Research in Chemistry
Year : 2019, Volume : 12, Issue : 2
First page : ( 112) Last page : ( 115)
Print ISSN : 0974-4169. Online ISSN : 0974-4150.
Article DOI : 10.5958/0974-4150.2019.00024.5

Development and Validation of UV Spectrophotometric Method for Estimation of Cyproheptadine hydrochloride

Prof. Patil Sushil D.*, Dugaje Tejashri, Dr. Kshirsagar Sanjay J.

MET's Institute of Pharmacy, MET League of Colleges, Bhujbal Knowledge City, Adgaon, Nashik, Savitribai Phule Pune University, Maharashtra, India

*Corresponding Author E-mail: sushilpharma@rediffmail.com

Online published on 15 May, 2019.


A new, simple, accurate and sensitive UV-spectrophotometric absorption correction method has been developed for estimation of cyproheptadine hydrochloride in bulk utilizing concept of standard addition. The method is based upon determination of cyproheptadine hydrochloride at 286nm, distilled water as a solvent. Linearity was observed in range of 2-10ug/ml for cyproheptadine hydrochloride. The correction coefficient value was found to near to1. All validation methods were validated as per ICH guideline.



Cyproheptadine HCl, UV-spectrophotometric, Method Development and Validation as per ICH guideline.


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