(34.200.218.187)
Users online: 1840    [ij] [ij] [ij] 
Email id
 

Asian Journal of Research in Chemistry
Year : 2018, Volume : 11, Issue : 6
First page : ( 871) Last page : ( 875)
Print ISSN : 0974-4169. Online ISSN : 0974-4150.
Article DOI : 10.5958/0974-4150.2018.00152.9

New, Sensitive, Simple and Validated Stability Indicating TLC/Densitometric Method for the Estimation of Anti-Ulcer Drug Mesalamine in Bulk and Tablet Formulation

Khan Mohammad Mojeeb Gulzar1,*, Shirkhedkar Atul Arun2

1Assistant Professor, Department of Pharmaceutical Chemistry, R. C. Patel Institute of Pharmaceutical Education and Research, Karwand Naka, Shirpur, Dist: Dhule (MS) India, 425405

2Professor, Department of Pharmaceutical Chemistry, R. C. Patel Institute of Pharmaceutical Education and Research, Karwand Naka, Shirpur, Dist: Dhule (MS) India, 425405

*Corresponding Author E-mail: mujeebgulzar@gmail.com

Online published on 3 July, 2019.

Abstract

Mesalamine is use in the treatment of ulcerative colitis (a health disorder which causes swelling and sores in the lining of the colon [large intestine] and rectum) and also to improve ulcerative colitis symptoms. Solubility of drug in volatile solvent is important criteria for development of TLC method. The presented work explains the development and validation of TLC/Densitometric method for Mesalamine by using accurate solvent for solubility of Mesalamine. Precoated TLC aluminium plates were utilized as a stationary phase and mobile phase composed of Toulene: Methanol: Triethylamine: (05: 05: 0.1 v/v/). Densitometric analysis for the Mesalamine is carried out at λmax 325. Method validation was performed as per ICH Guidelines. Stress degradation study was done under various reaction conditions. This system was found to give compact spots for Mesalamine at Rf value 0.63 ± 0.02. The data of linear regression analysis of Mesalamine signify a good linear relationship over the range of 400–2400 ng/band. LOD and LOQ found 37.66 and 114.14 ng for Mesalamine indicates sensitivity of the method. Resulted stress drugs were analyzed with the developed TLC/densitometry method. HPTLC method for Mesalamine has been developed by using proper volatile solvent as it is need of study. Statistical analysis verifies that the method is repeatable and selective for the determination of Mesalamine drug in bulk and in Pharmaceutical formulation. As the method could effectively separate the drug from its degradation products, it can be considered as a stability-indicating method.

Top

Keywords

Mesalamine, TLC, Densitometry, Validation, Stability Indicating.

Top

  
║ Site map ║ Privacy Policy ║ Copyright ║ Terms & Conditions ║ Page Rank Tool
435,453,524 visitor(s) since 30th May, 2005.
All rights reserved. Site designed and maintained by DIVA ENTERPRISES PVT. LTD..
Note: Please use Internet Explorer (6.0 or above). Some functionalities may not work in other browsers.