(3.238.204.31)
Users online: 2967    [ij] [ij] [ij] 
Email id
 

Asian Journal of Research in Chemistry
Year : 2018, Volume : 11, Issue : 4
First page : ( 755) Last page : ( 762)
Print ISSN : 0974-4169. Online ISSN : 0974-4150.
Article DOI : 10.5958/0974-4150.2018.00133.5

Stability Indicating HPTLC Method for Estimation of Metoprolol Tartrate in Bulk and in Pharmaceutical Formulation

Dadge Shailesh1, Dhale Chaitali1, Yadav Savita2, Rao Janhavi2,*

1Department of Quality Assurance Techniques, Bharati Vidyapeeth University, Poona College of Pharmacy, Erandwane, Pune-411038, India

2Department of Pharmaceutical Chemistry, Bharati Vidyapeeth University, Poona College of Pharmacy, Erandwane, Pune-411038, India

*Corresponding Author E-mail: raojanhavi@rediffmail.com

Online published on 24 October, 2018.

Abstract

In the present study a rapid specific economical HPTLC method has been developed for determination of Metoprolol tartrate in bulk and pharmaceutical dosage form. Chromatographic separation was achieved on aluminium plates precoated with silica gel 60 F254 aluminium plates with mobile phase of chloroform: methanol: ammonia 9: 1: 0.05 (v/v/v). Detection was performed densitometrically at 225 nm using Camag TLC scanner. The Rfof Metoprolol tartrate found to be 0.26±0.02 and linearity was found in the concentration range of 200–1200 ng/spot with correlation coefficient (R2) of 0.998. The method was validated as per ICH guidelines for precision, recovery and robustness. Metoprolol was subjected to acid and alkaline hydrolysis, oxidation, photochemical and thermal degradation as per ICH guidelines. Method developed was stability indicating. Statistical analysis proves that the method is suitable for the analysis of Metoprolol tartrate in bulk drug and in pharmaceutical formulations without any interference from the excipients.

Top

Keywords

Metoprolol tartrate, Thin layer chromatography, Stability indicating, Degradation, Validation, ICH guidelines.

Top

  
║ Site map ║ Privacy Policy ║ Copyright ║ Terms & Conditions ║ Page Rank Tool
520,535,981 visitor(s) since 30th May, 2005.
All rights reserved. Site designed and maintained by DIVA ENTERPRISES PVT. LTD..
Note: Please use Internet Explorer (6.0 or above). Some functionalities may not work in other browsers.