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Asian Journal of Research in Chemistry
Year : 2017, Volume : 10, Issue : 4
First page : ( 557) Last page : ( 565)
Print ISSN : 0974-4169. Online ISSN : 0974-4150.
Article DOI : 10.5958/0974-4150.2017.00092.X

Development and Method Validation of RP-HPLC For Simultaneous Determination of Pregabalin and Methylcobalamin in Pure and Pharmaceutical Dosage Form

Sreekanth D1, Ramya P.1, Vishwanadham Y2, Vanitha R3

1Department of Pharmaceutical Analysis, Palamuru University, Mahaboobnagar, TS, India.

2Department of Pharmaceutical Chemistry, Vishnu institute of Pharmaceutical education &Research(VIPER), Narsapur, Medak-TS, India.

3Department of Pharmaceutics, TRR college of Pharmacy, Hyderabad.

*Corresponding Author E-mail: vishwanadham.y@gmail.com

Online published on 17 October, 2017.


To develop a simple, sensitive, precise and accurate RP-HPLC method was developed for the quantitative estimation of Pregabalin and Methylcobalamin in bulk drug and the most common from delivery of drugs oral, sublingual, injection pharmaceutical dosage forms. This method was Pregabalin and Methylcobalamin was freely soluble in ethanol, methanol and sparingly soluble in water. The %RSD values were within 2 and the method was found to be precise. The results expressed in Tables for RP-HPLC method was promising. The RP-HPLC method is more sensitive, accurate and precise compared to the Spectrophotometric methods. This method can be used for the routine determination of Pregabalin and Methylcobalamin in bulk drug and in Pharmaceutical dosage forms.



RP-HPLC, Pregabalin, Methylcobalamin, Validation.


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