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Asian Journal of Pharmacy and Technology
Year : 2019, Volume : 9, Issue : 2
First page : ( 93) Last page : ( 98)
Print ISSN : 2231-5705. Online ISSN : 2231-5713.
Article DOI : 10.5958/2231-5713.2019.00016.3

Albizzia lebbeck extract mediated synthesis of Zinc Oxide Nanoparticles and study of its In-vitro Anti-diabetic and Anti-oxidant activity

Argade Pranita A.*, Bhutkar Mangesh A., Magdum Chandrakant S.

Department of Pharmaceutics, Rajarambapu College of Pharmacy, Kasegaon, Tal-Walwa, Dist-Sangli-415404

*Corresponding Author E-mail: argade.pran116@ gmail.com

Online published on 3 July, 2019.

Abstract

The present study aims to green synthesis of zinc oxide nanoparticle using Albizzia lebbeck aqueous bark extract and study of it's in vitro anti-diabetic and anti-oxidant activity. In the present work, green synthesized ZnO NPs, in vitro anti-diabetic activity was evaluated by several methods such as inhibition of α-amylase enzyme and Glucose uptake by yeast cell. In amylase enzyme activity, maximal amylase inhibition of 73.33%, 54.66% and 46.66% was exhibited by A.lebbeck, ZnO NPs and Acarbose respectively with IC50 values of 4.91, 9.61 and 3.91. The results revealed that the Acarbose possess potent antidiabetic activity than A.lebbeck bark extract and their synthesized ZnO nanoparticles. In the assay of glucose uptake by yeast cell it was observed that the uptake of glucose by yeast cells take place in a dose dependent manner and is directly propotional to the sample concentration. The synthesized ZnO NPs promoted glucose uptake of 89.16% at a concentration of 1μg/ml which is higher than that exhibited by A.lebbeck bark extract and standard Metformin. 62.5% and 94.16% respectively at the same concentration. The in vitro anti-oxidant activity was evaluated by various methods such as DPPH radical scavenging assay and reducing power ability assay. Synthesized ZnO nanoparticles showed more potent DPPH scavenging activity with inhibition 66.66% and IC50 value of 7.038. In reducing power assay, The study revealed that increasing absorbance value of standard Ascorbic acid indicated higher reducing power ability of Ascorbic acid as compared to A.lebbeck bark extract and their synthesized ZnO NPs.

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Keywords

Zinc oxide nanoparticle, Green synthesis, α-amylase, Glucose uptake by yeast, DPPH scavenging activity.

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