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Asian Journal of Pharmacy and Technology
Year : 2018, Volume : 8, Issue : 3
First page : ( 145) Last page : ( 148)
Print ISSN : 2231-5705. Online ISSN : 2231-5713.
Article DOI : 10.5958/2231-5713.2018.00023.5

Development and Validation of RP-HPLC Method for Simultaneous Estimation of Saxagliptin and Dapagliflozin in Tablets

More Sayali S., Sonawane Sandeep S.*, Chhajed Santosh S., Kshirsagar Sanjay J.

Department of Pharmaceutical Analysis, MET's Institute of Pharmacy, Bhujbal Knowledge City, Adgaon, Nashik-422 003

*Corresponding Author E-mail: sandeeps.iop@gmail.com

Online published on 24 October, 2018.

Abstract

A simple, accurate, precise and selective RP-HPLC method was developed and validated for simultaneous estimation of Saxagliptin (SAXA) and Dapagliflozin (DAPA) in tablet dosage form. Both drugs were separated on Phenomenex Hyperclone C18 column (250 ×4.6 mm, 5μ) using methanol: 20 mM phosphate buffer (pH3.0) (70: 30, v/v) in an isocratic mode at flow rate of 1 mL/min. Chromatographic determination was carried out at wavelength of 225nm. SAXA and DAPA were eluted at 4.70 and 6.45min, respectively. The method was found linear in the range of 2–12 μg/mL and 4–24 μg/mL for SAXA and DAPA, respectively. The method was validated for linearity, accuracy, precision and selectivity. The developed method can be used for routine analysis of SAXA and DAPA tablets.

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Keywords

Dapagliflozin, Saxagliptin, HPLC, Analytical Method Validation.

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