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Asian Journal of Pharmacy and Technology
Year : 2015, Volume : 5, Issue : 3
First page : ( 175) Last page : ( 181)
Print ISSN : 2231-5705. Online ISSN : 2231-5713.
Article DOI : 10.5958/2231-5713.2015.00025.2

Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Irbesartan and Atorvastatin in Synthetic Mixture

Paras Virani1,2,*, Rajanit Sojitra1, Bhadresh Savaj1, Hashumati Raj1, Vineet Jain1, Kalpesh Patel1, Vishnu Sutariya1

1Quality Assurance Department, Shree Dhanvantary Pharmacy College, Kim, Surat

2Research Scholar 2015, Gujarat Technological University, Gujarat

*Corresponding Author E-mail: parasvirani@gmail.com

Online published on 29 January, 2016.

Abstract

A simple, accurate, rapid and precise reverse phase high performance liquid chromatographic method was developed for simultaneous estimation of Irbesartan and Atorvastatin in synthetic mixture. Inertsil C18, 150 mm x 4.6 mm, 5 μm particle size in gradient mode with mobile phase Acetonitrile: 0.1% Formic acid (40: 60 v/v) and pH adjusted to 3.5 ± 0.1 with orthophosphoric acid was used. The flow rate was 1.0 ml/min and absorbance of individual component was measured at 262 nm. The retention times of Irbesartan and Atorvastatin were found to be 3.993 and 7.733 min, respectively. Linearity for Irbesartan and Atorvastatin was in the range of 400 -800 and 50–100μg/ml with correlation coefficient values 0.9995and 0.9994, the percentage recovery obtained was 99.88and 99.70%, respectively.

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Keywords

Irbesartan, Atorvastatin, RP-HPLC, Method validation.

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