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Asian Journal of Pharmacy and Technology
Year : 2020, Volume : 10, Issue : 3
First page : ( 127) Last page : ( 133)
Print ISSN : 2231-5705. Online ISSN : 2231-5713.
Article DOI : 10.5958/2231-5713.2020.00022.7

Standardization and phytochemical screening analysis for herbal extracts: Zingiber officinalis, Rosc., Curcuma longa Linn., Cinnamonum zeylanicum Nees., Piper longum, Linn., Boerhaavia diffussa Linn.

Sharma Ruchika*, Kumari Neha**, Ashawat M.S, Verma C.P.S

Sri Sai College of Pharmacy, Badhani, Pathankot

*Corresponding Author E-mail: ruchikasharma471@gmail.com

**neha91sn@gmail.com

Online published on 16 September, 2020.

Abstract

The aim of the study was to standardization and phytochemical screening analysis for Herbal Extracts: Zingiber Officinalis, Rosc., Curcuma Longa Linn., Cinnamonum Zeylanicum Nees., Piperlongum, Linn., Boerhaavia Diffussa Linn. Phytochemical analysis of extracts, preformed identification tests of extracts and it is the qualitative analysis. The study involved chromatographic fingerprinting of five drug extracts; cinnamon (Cinnamomum Zeylanicum Nees), long pepper (Piper Longum, Linn.), turmeric (Curcuma Longa Linn), punarnava (Boerhaavia Diffussa, Linn.) and ginger (Zingiber Officinalis, Rosc.) for standardization followed by preparation of tablets by direct compression methods. Thin layer chromatography (TLC) and High performance liquid chromatography (HPLC) of extracts were performed and it is the quantitative and qualitative analysis. Phytochemical screening test of all five extracts; ginger, cinnamon, curcumin, long pepper and punarnava for alkaloid, carbohydrates, glycocides, cardio glycosides, saponin glycosides, tannin and steroids test were present. Thin Layer Chromatography (TLC) was performed to all extracts: Rf value 0.272 with mobile phase toulene: ethyl acetate (93:7), Rf value 0.40 with dichloromethane: methanol (60:40), Rf value 0.466 with petroleum ether: alcohol (70:30), Rf value 0.396 with toulene: ethyl acetate (93:7) and Rf value 0.333 with benzene: acetic acid (75:25) of Zingiber Officinale, Rosc., Cinnamonum Zeylanicum, Nees., Piper Longum, Linn., Curcuma Longa, Linn. and Boerhaavia Diffussa, Linn. respectively. high Performance Liquid Chromatography (HPLC) was used for quantitative purpose of all five extracts. The quantitative separation was confirmed on Shimadzu equipment. The extracts in flow rate of 1ml/min at the wavelength 282nm, 425nm, 221nm, 345nm and 233nm of Ginger, Turmeric, Cinnamom, Long Pepper and Punarnava respectively.

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Keywords

Phytochemical Screening, HPLC, TLC, Standardization, Extracts (Ginger, Cinnamon, Curcumin, Long Pepper and Punarnava).

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