Evaluation of anti-inflammatory activity of Hydroalcoholic extract of Ananas cosmosus fruit peel by HRBC membrane stabilisation.
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To evaluate invitro anti-inflammatory activity of hydroalcoholic extract of Ananas cosmosus fruit peel by HRBC membrane stabilisation.
Hydroalcoholic extract was prepared by soxhlet extraction and thereafter subjected for membrane stabilisation assay to evaluate anti-inflammatory property. 10% human red blood cell suspension was subjected to hyptonicity induced hemolysis and inhibition of membrane damage by the extract was compared to the standard drug diclofenac sodium.
Hypotonicity induced HRBC membrane lysis was inhibited by hydroalcoholic extract of Ananas cosmosus fruit peel in a concentration dependent manner. Hydroalcoholic extract showed 72.86% protection of HRBC membrane at 250μg/ml and showed significant membrane stabilisation compared with standard drug diclofenac sodium at the same concentrations.
Ananas cosmosus fruit peel extract showed appreciable HRBC membrane stabilisation and may have potential anti-inflammatory property. Further analysis is to be carried out to isolate active chemical constituent responsible for anti-inflammatory activity and its mechanism involved.
Ananas cosmosus, HRBC membrane, anti-inflammatory, hemolysis, soxhlet.