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Asian Journal of Pharmaceutical Research
Year : 2016, Volume : 6, Issue : 1
First page : ( 15) Last page : ( 20)
Print ISSN : 2231-5683. Online ISSN : 2231-5691.
Article DOI : 10.5958/2231-5713.2016.00002.7

Simultaneous UV Spectrophotometric Methods for Estimation of Carvedilol and Hydrochlorothiazide in Bulk and Tablet Dosage Form

Mali Audumbar1,*, Kekan Vikas2, Dongare Rohan2, Gholve Sachin2, Bathe Ritesh1

1Department of Pharmaceutics, Sahyadri College of Pharmacy, Methwade, Sangola-413307, Solapur, Maharashtra, India

2Department of Quality Assurance, Channabasweshwar Pharmacy College, Latur. Dist., Latur-413512, Maharashtra, India

*Corresponding Author E-mail: maliaudu442@gmail.com

Online published on 2 April, 2016.

Abstract

Two simple, precise, economical, fast and reliable two UV methods have been developed for the simultaneous estimation of Carvediloland Hydrochlorothiazide in bulk and pharmaceutical dosage form. Method A is Absorbance maxima method, which is based on measurement of absorption at maximum wavelength of 290 nm and 271 nm for Carvedilol and Hydrochlorothiazide respectively. Method B is area under curve (AUC), in the wavelength range of 260–308 nm for Carvedilol and 2246-292 nm for Hydrochlorothiazide. Linearity for detector response was observed in the concentration range of 5–25μg/ml for Carvedilol and 5–25 μg/ml for Hydrochlorothiazide. The accuracy of the methods was assessed by recovery studies and was found to be 100.13% and 102.28% for Carvedilol and 99.04% and 99.89% Hydrochlorothiazide by using method A and B respectively. The developed method was validated with respect to linearity, accuracy (recovery), precision and specificity. The results were validated statistically as per ICH Q2 R1guideline and were found to be satisfactory. The proposed methods were successfully applied for the determination of for Carvedilol and Hydrochlorothiazide in commercial pharmaceutical dosage form.

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Keywords

Carvedilol, Hydrochlorothiazide, Simultaneous estimation, Accuracy, Absorbance maxima method, Area under curve.

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