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Asian Journal of Pharmaceutical Research
Year : 2015, Volume : 5, Issue : 2
First page : ( 108) Last page : ( 113)
Print ISSN : 2231-5683. Online ISSN : 2231-5691.
Article DOI : 10.5958/2231-5691.2015.00016.7

Development and Validation of Advance Simultaneous Equation Methods for Lidocaine Hydrochloride and Clotrimazole in Ear Drop

Patel Bhavisha G.*, Mr. Gheewala Nirav, Raj Hasumati A.

Shree Dhanvantary College of Pharmacy, Kim, Surat, Gujarat, India

*Corresponding Author E-mail: bhavisha.patel65@yahoo.com

Online published on 29 October, 2015.

Abstract

A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of Lidocaine Hydrochloride and Clotrimazole in Ear Drop by Advance simultaneous equation method. A zero order spectrum was recorded and convert in ratio second order derivative. The Lidocaine Hydrochloride shows max absorbance at 243.20 nm and Clotrimazole show max absorbance at 225 nm. The method was found to be linear (r2>0.999) in the concentration range of 20–100μg/ml for Lidocaine Hydrochloride and 10–50μg/ml for Clotrimazole. The limit of determination was 0.2498μg/ml and 0.0837μg/ml for Lidocaine Hydrochloride and Clotrimazole, respectively. The limit of quantification was 0.7570μg/ml and 0.2537μg/ml for Lidocaine Hydrochloride and Clotrimazole, respectively. The accuracy of this method was evaluated by recovery studies and good recovery result was obtained greater than 99%. The method was successfully applied for simultaneous determination of Lidocaine Hydrochloride and Clotrimazole in Ear Drop.

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Keywords

Lidocaine Hydrochloride, Clotrimazole and Advance simultaneous equation method.

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