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Asian Journal of Pharmaceutical Research
Year : 2020, Volume : 10, Issue : 3
First page : ( 183) Last page : ( 187)
Print ISSN : 2231-5683. Online ISSN : 2231-5691.
Article DOI : 10.5958/2231-5691.2020.00032.5

Screening phytochemical, antioxidant activity and vitamin c assay from bungo perak-perak (Begonia versicolar irmsch) leaves

Abriyani Ermi*, Fikayuniar Lia

Department of Pharmacy, Buana Perjuangan University of Karawang, West Java, Indonesia

*Corresponding Author E-mail: ermi.abriyani@gmail.com

Online published on 16 September, 2020.

Abstract

The study of screening phytochemical and activity antioxidant has been done and this is the first report on Bungo Perak-perak (Begonia versicolar Irmsch) leaves about screening phytochemical, antioxidant activity and vitamin C assay. The extraction using maceration method on Begonia versicolar Irmsch leaves with three type solvent are n-hexane, ethyl acetate and methanol. Antioxidant activity assay from n-hexane, ethyl acetate, and methanol extracts at various concentration (31.25μg/ml, 62.5μg/ml, 125μg/ml) using the DPPH (1,1-diphenyl-2-dipicryl-hydrazin/DPPH•) methods and as reference solution is ascorbic acid. Absorbance was measured at a wavelength of 515nm in a uv-visible spectrophotometer. Determination of vitamin C assays was tested by iodometry methods. The results of phytochemical from Begonia versicolar Irmsch are indicated the sample had contains alkaloids, flavonoids, phenolic, terpenoids, steroids, carbohydrate, saponin and reducings sugar. The results of activity antioxidants are ethyl acetate 69.82μg/ml, and methanol extracts 8.67μg/ml. Result of vitamin C assays from Begonia versicolar Irmsch are 3.256mg from 20grams sample. This research was showed Begonia versicolar Irmsch can be a good source of antioxidants and have vitamin C and good of phytochemicals that supports traditional herbal medicine.

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Keywords

Begonia versicolar Irmsch, Phytochemicals, Antioxidants activity, Vitamin C assay.

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