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Asian Journal of Pharmaceutical Analysis
Year : 2019, Volume : 9, Issue : 4
First page : ( 229) Last page : ( 231)
Print ISSN : 2231-5667. Online ISSN : 2231-5675.
Article DOI : 10.5958/2231-5675.2019.00039.5

Development and Validation of Analytical Method by RP-HPLC and Forced Degradation Studies of Tioconazole Drug

Surse Sunita N*, Patil Sushil D**, Deshmukh Kunal R, Kshirsagar Sanjay J

Pharmaceutical Quality Assurance Technique, MET's Institute of Pharmacy, Bhujbal Knowledge City, Adgoan, Nashik, Savitribai Phule Pune University

*Corresponding Author E-mail: sunitasurse@gmail.com

**sushilpharma@rediffmail.com

Online published on 31 December, 2019.

Abstract

A Simple, Sensitive, Precise and Specific high performance liquid chromatography method was developed and validated for the determination of tioconazole drug. The separation was carried out by using a mobile phase consisting of methanol: phosphate buffer (pH3) in ratio of 95: 05. The column used was cosmosil C18 (250 mmx 4.6 ID) Flow rate of 0.9 ml/min. using UV detection at 220 nm. The retention time of tioconazole was found to be 4.67 min. resp. A forced degradation study of tioconazole in its API form was conducted under the condition of hydrolysis, oxidation, thermal and photolysis. The methods were validated by following the Analytical performance parameters suggested by the International Conference on Hormonaization (ICH).

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Keywords

Dual wavelength, absorbance, Tioconazole.

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