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Asian Journal of Pharmaceutical Analysis
Year : 2019, Volume : 9, Issue : 4
First page : ( 224) Last page : ( 228)
Print ISSN : 2231-5667. Online ISSN : 2231-5675.
Article DOI : 10.5958/2231-5675.2019.00038.3

Simultaneous Estimation of Candesartan and Hydrochlorothiazide in Combined Tablet Dosage Form by RP-HPLC

Pappula Nagaraju*, Ravichandra S., Sindhura S. Lakshmi, Rani P. Asha, Madhuri V. Seshu, Ajay V., Reddy P. Narsi

Department of Pharmaceutical Analysis, Hindu College of Pharmacy, Amravathi Road, Guntur-522002, Andhra Pradesh, India

*Corresponding Author E-mail: pappulanagaraju@gmail.com

Online published on 31 December, 2019.

Abstract

The study describes method development and subsequent validation of RP-HPLC method for simultaneous estimation of candesartan and hydrochlorothiazide in combined tablet dosage forms. Chromatographic separation was achieved on a Hypersil BDS C8 column (250 mm x 4.6 mm, 5 μm) using a mobile phase consisting of (40: 60 v/v) acetonitrile: triethylamine (0.02%) at a flow rate of 1 mL/min. The detection wavelength is 262 nm. The retention times of candesartan and hydrochlorothiazidewere found to be 2.449 min and 4.895 min respectively. The developed method was validated as per ICH guidelines. The developed and validated method was successfully used for the quantitative analysis of candesartan and hydrochlorothiazidein tablet dosage forms.

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Keywords

RP-HPLC method, Candesartan, Hydrochlorothiazide, Validation.

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