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Asian Journal of Pharmaceutical Analysis
Year : 2019, Volume : 9, Issue : 4
First page : ( 215) Last page : ( 218)
Print ISSN : 2231-5667. Online ISSN : 2231-5675.
Article DOI : 10.5958/2231-5675.2019.00036.X

Development and Validation of Q-absorbance Ratio method for Simultaneous Estimation of Teniligliptin hydrobromide and Metformin HCl in Multicomponent Dosage Form

Gadge Sonali S.1,*, Nakod Ashish D.2, Wasnik Vaishali P.2, Gatkine Tilottama M.2, Zile Shrutika S.2, Mohije Najuka H.2, Salode Vikrant L.2

1Assistant Professor, P. R. Patil Institute of Pharmacy, Talegaon (S.P.), Wardha-442202

2P. R. Patil Institute of Pharmacy, Talegaon (S.P.), Wardha-442202

*Corresponding Author E-mail: sonaligadge94@gmail.com

Online published on 31 December, 2019.

Abstract

The main aim of this study was focuses on the development and validation of q-absorbance ratio method for simultaneous estimation of Teniligliptin hydrobromide and Metformin HCL in multicomponent dosage form with the main and important objective was to develop and validate the method for accuracy results. A simple, accurate, sensitive and precise UV-spectrophotometric (Q-Absorbance ratio) method for the simultaneous estimation of Teniligliptin Hydrobromide and Metformin hydrochloride in multicomponent dosage form was developed and validated for linearity and range, accuracy, precision, limit of detection and limit of quantitation. Q-Absorbance ratio method is employed for simultaneous estimation of Teniligliptin hydrobromide and Metformin hydrochloride from multi component dosage forms. In this method, the absorbance was measured at 241.63 nm for Teniligliptin hydrobromide and 231.65 nm for Metformin hydrochloride. Linearity for detector response was observed in range of 0.5–2.5 μg/ml and 12.5–62.5μg/ml for Teniligliptin hydrobromide and Metformin HCl respectively. Recovery studies confirmed accuracy of proposed method and results were validated as per ICH guidelines. The % RSD values were found to be less than 2 indicates good precision and reproducibility of the method. The method can be used for routine quality control of pharmaceutical formulation containing Teniligliptin hydrobromide hydrate and Metformin hydrochloride.

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Keywords

Teniligliptin, Metformin, UV Spectrophotometry, Q-Absorbance ratio method, method validation.

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